Composite

Part:BBa_K2423002:Design

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)


UGTCs2 under the control of a constitutive promoter (BBa_J23106)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 593
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 766
    Illegal AgeI site found at 1121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made a complete device including promoter and RBS. We also included a 6x his tag for easy purification of the expressed protein.


Source

The gene of interest had previously been worked on by Moraga et al. (2004) (1), where they were able to clone the cDNA of UGTCs2. They published the sequence that they used on GenBank with AY262037 as the accession number (2).

References

1. Moraga AR, Nohales PF, Pérez JAF, Gómez-Gómez L. Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas. Planta. 2004 Oct 1;219(6):955–66.

2. Crocus sativus glucosyltransferase 2 (GLT2) mRNA, complete cds. 2003 Nov 1; Available from: http://www.ncbi.nlm.nih.gov/nuccore/AY262037.1